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Frequently Asked Questions for BIOXYTECH®
8-OHdG (8-Hydroxy-2'-deoxyguanosine) ELISA
Catalog Number: 21026
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How long does the test take to perform?
Reagent Preparation: Approximately 10 minutes Assay Procedure: Approximately 2 1/2 hours

What samples may be tested for 8-OhdG?
Tissue, plasma, serum and urine may be tested with this assay kit.

How are samples prepared prior to testing?
Pretreatment of urine samples is not required, unless they are turbid or contain precipitate. In this case, centrifugation is required at 2000 g for 10 minutes. Serum samples must be filtered using ultra-filtration, with a 10,000 molecular weight cut off. Both plasma and tissue require that DNA be extracted and digested. This may be done by several methods, based on the researcher's preference.

Is a standard needed to perform the assay?
Yes; standards range from 0.5 ng/ml to 200.0 ng/ml. These come ready to use in individual vials, no mixing or dilutions required.

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