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Frequently Asked Questions for BIOXYTECH®
Lactoferrin ELISA
Catalog Number: 21015
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How long does the test take to perform?
30 minutes to bring reagents to ambient temperature. 10 minutes for sample and reagent preparation. 1 hour for standard and sample incubation. 1 hour for anti-LTF incubation. 15 minutes for avidin-HRD incubation. 10 minutes for OPD reaction. Total test time is 3 hours. Hands-on time is 30 minutes or less.

What is the sensitivity of the Lactof-EIA method?
The detection limit of the assay is 1.0 ng/ml.

What samples can be tested for lactoferrin?
Serum, plasma, urine, broncho-aveolar lavage, spinal cord fluid and supernatants from cell culture.

How are samples prepared prior to testing?
No special sample preparation needs to occur other than diluting samples in sample buffer when necessary.

What is the normal concentration of lactoferrin in samples?
This is not known.

What is the stability of the reaction?
Stop solution is added to the final reaction mixture which stabilizes the reaction for 15 to 20 minutes.

What control can be used to assess the correct performance of the kit?
Measurements of the standards that are provided in the kit can be used as samples to check proper assay performance. The optical densities of these results can be compared with the optical densities that should be obtained with the standards as stated in the package insert.

What is the shelf life of the lactoferrin kit?
12 months after the date of manufacture. All components if stored correctly can be used until the expiration of the kit.

10. How is lactoferrin concentration calculated in the procedure?
A standard curve is performed by plotting concentration vs. absorbance. The absorbance of the unknown sample is plotted on the standard curve to find its concentration.

What are the advantages of the lactoferrin method?
Standard curve reagents are provided. Most of the reagents are ready to use. Procedure can be automated. There are minimal operator steps. Most of the time spent on the procedure is incubation.

What are the competitive advantages of the lactoferrin assay?
This is a unique assay. There are no other commercially available products for this assay on the market.

What are the potential interferences/ pitfalls of this assay?
The immunoassay is specific and sensitive. No interferences have been apparent.

Does the Lactoferrin EIA cross-react in mouse and rat models?
Cross-reactivity remains unknown at this time.


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